Observed genotypic ratios at the peak marker from Scarb1I179N breeding mice.

Homozygous D2 alleles shown by ‘wt/wt’; ‘Het’ indicates a single copy of B6 carrying the ENU mutation and a D2 allele, ‘m/m’ indicates homozygous B6 mutants carrying both mutated alleles. Backcross population: Mice that were heterozygous for the Scarb1 mutation (B6:D2 derived from the backcross BC1 population) were used for further mapping by backcrossing a second time to D2 (BC2 giving rise to 24 litters. Genotypes of the progeny are not significantly different from the expected frequency. Intercross population: Nine litters were generated by inter-crossing animals that were heterozygous (from the backcross population BC2) segregating at the Scarb1 mutation. The ratios are not significantly different from Hardy-Weinberg equilibrium.