Bacterial diversity sequencing of perennial sorghum silage

The aim of this study was to evaluate the effects of lactic acid bacteria and cellulase on perennial sorghum silage by analyzing fermentation parameters, chemical composition and bacterial community. When cutting at flowering stage, control treatment (CK) without additives, cellulase treatment (CE), Lactobacillus brucei treatment (LAB) and cellulase + Lactobacillus brucei treatment (LC) were performed respectively. The silage performance and bacterial community were measured 45 days after mowing at different densities. Compared with CK, the other treatment groups had lower pH. The addition of Lactobacillus brucei increased the contents of LA and AA. After fermentation, the contents of WSC, CP and EE in CK were lower than those in LAB, CE and LC, and the contents of NDF and ADF were higher than those in LAB, CE and LC. Compared with CK, the addition of Lactobacillus brucei significantly reduced bacterial diversity, while increasing Lentilactobacillus and decreasing Proteobacteria. In addition, with the progress of fermentation, the abundance of Amino acid metabolism in LAB and LC groups was significantly higher than that in CE and CK groups. The abundances of beta-Lactam resistance, Flagellar assembly, Ascorbate and aldarate metabolism were significantly reduced. In summary, the addition of LAB and LC regulated the fermentation parameters, bacterial community and metabolic pathway of silage, and then improved the fermentation quality of perennial sorghum.