Modulation of DNA methyltransferases (DNMTs) in Spodoptera frugiperda (Sf9) cells following AcMNPV infection and its effects on the virus-cell interaction

In this study, we examined the gene expression profiles of Sf9 insect cells following infection with baculovirus, utilizing reverse transcription quantitative PCR (RT-qPCR) to analyze how the host cellular machinery responds at the transcriptional level to viral invasion. Our findings highlight the intricate regulatory mechanisms in place during virus replication. To further understand the relationship between the host response and viral propagation, we also quantified the dynamics of baculovirus replication within the Sf9 cells using quantitative PCR (qPCR). This dual approach provides a comprehensive view of both the efficiency of viral replication and the accompanying host transcriptional changes.
, , # Modulation of DNA methyltransferases (DNMTs) in Spodoptera frugiperda (Sf9) cells following AcMNPV infection and its effects on the virus-cell interaction

Dataset DOI: [10.5061/dryad.pk0p2nh1j](10.5061/dryad.pk0p2nh1j)

## Description of the data and file structure

**File: data.txt**

Description: Includes a text file of tables.

To analyze gene expression levels in AcMNPV-infected and control uninfected Sf9 cells, total RNA was extracted from cells at 4, 8, 16, 24 and 48 hpi. Also, to determine the effect of 5-AZA on the expression of immune-related genes, RNA samples were extracted at 24 and 48 h post AcMNPV infection. Total RNA was extracted by Tri- Reagent™ according to the manufacturer's instructions (Molecular Research Center Inc.). RNA samples were treated with DNase I and incubated at 37 °C for 10 min followed by heat inactivation at 75 °C for 10 min. RNA concentrations were measured using an Epoch microplate spectrophotometer (BioTek) and integrity was ensured through analy...,