scRNA-seq analysis of hepatocellular carcinoma with microvascular invasion.

Microvascular invasion (MVI) is a significant pathological feature of hepatocellular carcinoma (HCC) associated with poor outcomes, early recurrence and intrahepatic metastasis in post-surgical resection and transplant patients. However, the intricate tumor microenvironment (TME) and transcriptional programs underlying MVI in HCC remain poorly understood. We conducted single-cell RNA sequencing of 46,789 individual cells from 10 samples of MVI+ (MVI present) and MVI- (MVI absent) HCC patients. By examining the cellular and molecular features associated with MVI, we conducted comprehensive analyses and validated key findings.