scRNA-seq analysis of hepatocellular carcinoma with microvascular invasion.

Microvascular invasion (MVI) is a significant pathological feature of hepatocellular carcinoma (HCC) associated with poor outcomes, early recurrence and intrahepatic metastasis in post-surgical resection and transplant patients. However, the intricate tumor microenvironment (TME) and transcriptional programs underlying MVI in HCC remain poorly understood. We conducted single-cell RNA sequencing of 46,789 individual cells from 10 samples of MVI+ (MVI present) and MVI- (MVI absent) HCC patients. By examining the cellular and molecular features associated with MVI, we conducted comprehensive analyses and validated key findings.微血管侵犯（Microvascular invasion, MVI）是肝细胞癌（hepatocellular carcinoma, HCC）的重要病理特征，与接受手术切除或肝移植的术后患者的不良预后、早期复发及肝内转移密切相关。然而，肝细胞癌中与微血管侵犯相关的复杂肿瘤微环境（tumor microenvironment, TME）及转录调控程序，目前仍未得到充分阐释。本研究对10例涵盖MVI阳性（MVI present）与MVI阴性（MVI absent）的肝细胞癌患者样本中的46789个单个细胞进行了单细胞RNA测序（single-cell RNA sequencing）。通过解析与微血管侵犯相关的细胞及分子特征，本研究开展了全面的分析，并对关键研究结果进行了验证。