Horizontal mitochondrial transfer from microenvironment increases glioblastoma tumorigenicity. Horizontal mitochondrial transfer from microenvironment increases glioblastoma tumorigenicity

Glioblastoma (GBM) cells acquire mitochondria from glial cells in vitro and in vivo. This transfer is accompanied by increased tumor-initiation capacity. Overall design: GFP+ SB28 tumor cells were co-cultured with CellTrace Violet-stained astrocytes from mito::mKate2 mice at a 1:1 ratio for 2 days. Tumors with or without exogenous mitochondria and donor astrocyte population were sorted based on CellTraceViolet-GFP-+mKate- (astrocyte), CellTraceViolet+GFP-mKate2-(mKate_neg) and CellTraceViolet+GFP-mKate2+(mKate_pos) from 3 biological replicates.