Summary of experiments testing the localisation of Xvelo proteins and mutants.

*eGFP-XveloFLΔ3 localisation was always very weak, and only discernable at very high gain settings during confocal microscopy. If localisation ability was strictly based on the criteria used for the other full length proteins or mutants (ie constant lower gain) they would probably all be scored as negative.N is the number of experiments using different batches of oocytes The number of oocytes displaying germ plasm localisation are scored with respect to the total number injected. For eGFP-XveloFL and eGFP-XveloSV the numbers shown are exclusively for experiments in which the mutants were also tested. Similar data were obtained for these proteins in a much larger number of experiments.