Sequential expression profile of cortical neuron differentiation from human induced pluripotent stem cells (iPSCs) by RNA-Seq

We aim to profile the dynamic changes of gene expression dynamics during cortical neuron differentiation from human iPSCs. We used RNA-seq to map open chromatins in iPSCs, neural stem cells (NSCs) at day 33 and day 41. We assembled gene expression profiles in iPSCs derived from fibroblasts (GM01835), and iPSC-differentiated cortical neural stem cells (NSCs) at day 33 and day 41 of neural induction (designated as iN-d33 for simplicity), and neurons at day 41 (iN-d41). At each stage (iPSC, iN-d33 or iN-d41), we included two cell culture replicates. We mapped the raw FASTA file against human g1000 transcriptome and counted the RPKM value of each gene using R-based scripts.