Dynamic redistribution of CTCF demarcates bifurcation of cytotoxic effector and memory precursor programs [Hi-C]

CTCF is known to mediate long range chromatin intearction and 3D genome organization. In CD8+ T cells responding to actue infection, CTCF is redistributed to induce the the cytotoxic program while using its insulation function to suppresss memory precursor fate. Overall design: Naive P14 TCR transgenic CD8+ T cells were adoptively transferred into CD45 disparate recipients followed by infection with LCMV Armstrong. On day 8 post infection, KLRG1+IL-7Ra- P14 cells were sort purified as effector CD8 T cells and used for in situ HiC analysis.