scRNA-seq + TCR-seq of mouse polyclonal CD4+ T cells prior to and after re-infection.

Naturally-acquired immunity to blood-stage malaria is associated with several effector CD4 + T subsets including germinal centre (GC) Tfh, Th1 and Tr1 cells. Children in malaria-endemic regions can experience repeated Plasmodium infections over short periods of time; yet the effect of reinfection on multiple co-existing effector and memory subsets remains unclear. Here, we tracked antigen-experienced polyclonal CD4+ T cells during Plasmodium re-infection in mice using scRNA-seq/TCR-seq. Overall design: Mice were infected with Plasmodium chabaudi chabaudi AS (PcAS) parasites and mice were administered with artesunate (intermittent artesunate therapy - IAT) at Day 7. At Day 27, mice were re-infected with PcAS parasites. CD4+ T cells were recovered at Day 30, and Day 3 after re-infection to undergo processing and generate scRNA-seq dataset.