Gene expression analysis of WT and ZIP13-depleted human mesenchymal stem cells

In order to identify the genes whose expression was perturbed by ZIP13, we performed a gene expression microarray analysis for the RNAs isolated from WT and ZIP13-depleted human mesenchymal stem cell. Duplicate experiments were performed for WT and ZIP13 knock-down human mesenchymal stem cells. Total RNA was isolated from tissues using Qiazol (QIAGEN) following the manufacturer's protocols.