OMAP-3 Organ Mapping Antibody Panel (OMAP) for Multiplexed Antibody-Based Imaging of Kidney with CODEX, v1.1

OMAP-3 was designed for CODEX (CO Detection by IndEXing) of fresh frozen human kidney samples<br>(https://doi.org/10.1038/s41596-021-00556-8, https://doi.org/10.1002/eji.202048891). The panel contains 24 antibodies<br>and the nuclear marker DAPI for image alignment and nuclear segmentation. Additional details on tissue preparation,<br>panel design, image analysis, and representative images can be found in accompanying publications<br>(https://doi.org/10.1016/j.kint.2021.08.033, https://doi.org/10.1016/j.xpro.2021.100747). This OMAP provides a spatial<br>context for all anatomical structures and most cell types present in the [ASCT+B kidney table<br>v1.1](https://doi.org/10.48539/HBM673.ZZDK.622). Additionally, the inclusion of tryptase and MARCKS allow profiling of<br>different regions in the glomeruli that are often affected by disease. Several cell types are identified by their<br>morphology, location in the kidney, and expression of Cytokeratin 7. The panel was designed to provide a survey of the<br>cell types found in the kidney and does not allow for granular phenotyping of immune cell populations. However, mast<br>cells, NK cells, T cells, and B cells are broadly classified by several markers included in this OMAP. The core and<br>essential protein biomarkers detailed here overlap (>40%) with a panel developed for multiplexed imaging of kidney<br>samples using imaging mass cytometry (IMC) (10.1172/jci.insight.129477).