Proteomics of venoms of Naja naja and Daboia russelii snakes

The current research advises the usages of variations in proteomics of venoms of Naja naja and Daboia russelii for identification of suspected snake venom samples seized by law enforcement agencies across the world, especially in India. Illegal trade of endangered species of snakes and their venoms is prohibited by Indian Wildlife Protection Act, 1972. There are provisions of fines and imprisonments if a person is found involved in such illegal activities. The Naja naja and Daboia russelii, both are endangered species and illegal trading of venoms of both these snakes is frequently encountered by various law enforcement agencies. But to frame charges under Wildlife Protection Law, the enforcement agencies are required to prove beyond any doubt that the seized material is venom illegally milked from these species. The unique proteomes of venoms can be helpful in confirm identification of snake species from which the venom is likely milked. The data is generated by LC-MS/MS (nano-Liquid Chromatography coupled with Q-Orbitrap mass spectrometr and ProteomeDiscoverer 2.2 software) of crude venoms of Naja naja and Daboia russelii.本研究提出利用印度眼镜蛇（Naja naja）与罗素蝰蛇（Daboia russelii）蛇毒蛋白质组的差异特征，对全球各地执法机构查获的疑似蛇毒样本进行鉴定，该方案在印度地区尤为适用。1972年施行的《印度野生动物保护法》明确禁止濒危蛇类及其蛇毒的非法贸易行为，参与此类非法活动的人员将被处以罚款或面临监禁处罚。印度眼镜蛇与罗素蝰蛇均属于濒危物种，各类执法机构时常查获该两类蛇的蛇毒非法交易案件。但依据《野生动物保护法》提起刑事指控时，执法机构需提供确凿无疑的证据，证明查获物品为从上述物种中非法采得的蛇毒。蛇毒独特的蛋白质组特征可有效辅助确认该蛇毒的来源蛇类物种。 本数据集通过液相色谱-串联质谱（LC-MS/MS，即纳米液相色谱联用Q-轨道阱质谱仪及ProteomeDiscoverer 2.2软件）分析印度眼镜蛇与罗素蝰蛇的粗制蛇毒后生成。