Substrate specificity of MZ0003 towards p-NP esters and acetyl esters.
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a: Rate was measured using the time-resolved spectrophotometric assay for detection of liberated p-nitrophenol group expressed per mg of protein. b: Activity was measured as the relative amount of acetyl group liberated per mg of protein by endpoint assay. Activities with each substrate are expressed relative to the activity with p-NP acetate, which was measured using both methods. All measurements were done at 35°C and represent the mean of three independent determinations with the standard deviation given as the error.
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2016-09-28



