Circular dichroism and DNA binding affinities.
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aNS DNA is non-specific DNA (5′-TGCAGGAATTCCAAGGTGAAGGTT);bE-box DNA used in CD is 5′-TGCAGGAACCACGTGGTGAAGGTT. For previously published Kd values obtained by fluorescence anisotropy, the same DNA sequence was used, except that the forward oligonucleotide was labeled at the 5′ end with 6-carboxyfluorescein (6-FAM);cfrom [23];dfrom [41]. Note that our CD data is presented for 10 µM monomeric protein concentrations, whereas Wendt et al. used 20 µM E47 monomer [41].
创建时间：
2015-12-02
