Disulfiram/Cu induce ferroptosis in triple-negative breast cancer cells

To further explore the mechanism of DSF/Cu in TNBC, we conducted transcriptome sequencing experiments in MDA-MB-231 cells in response to 24 h treatment with DSF/Cu (0.2 µM). RNA-seq data revealed that 874 genes were upregulated and 764 genes were downregulated in DSF/Cu treated cells. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that TNBC cells death induced by DSF/Cu wais related to MAPK signaling pathway, p53 signaling pathway, mitophagy signaling pathway and ferroptosis. MDA-MB-231 cells were seeded in 6-well plates and incubated with culture mediums. The next day, cells were treated with DMSO or DSF/Cu and incubated for 24 h. The cells of three wells were maxed into one repeat, and three repeats were performed for RNA-sequencing.