Stochastic Regression and Peak Delineation with Flow Cytometry Data

This data repository contains original files (fcs) of flow cytometry experiments. The data was used to demonstrate the use of stochastic regression to quantify subpopulations of cells that have distinctly different genome copies per cell within a heterogenous population of Escherichia coli (E. coli) cells. This new approach gives estimates of signal and noise, the former of which is used for analysis, and the latter is used to quantify uncertainty. By separating these two components, the signal and noise can be compared independently to evaluate measurement quality across different experimental conditions.