IVM and MOX concentrations in brain and brain-to-plasma ratio at increasing dose rates in Mdr1ab(−/−) and wild-type mice.

IVM or MOX was administered to Mdr1ab(−/−) mice (6 per dose rate) or to wild-type mice (3 per dose rate) at increasing doses below the LD50 to ensure a non-lethal effect of the administration. Drug concentrations were determined in brain and plasma after animals were sacrificed at 24 h post-treatment. Absolute brain accumulation was plotted against the plasma concentration to determine brain concentration at LD50, brain-to-plasma concentration ratio calculated and R2.aLD50 determined graphically from Figure 1.bLD50 for IVM and MOX determined from the literature [20], [21].cBrain concentration reached at LD50, determined graphically from Figure 3A.dBrain concentration reached at LD50, determined graphically from Figure 3B.eBrain-to-plasma concentration ratio calculated and R2 obtained from the slope of the linear relationship between brain concentration and plasma concentration, which quantifies blood-brain transport.