Cardiotrophin Like Cytokine Factor 1 (CLCF1) alleviates bone loss and osteoclastogenesis in osteoporosis mouse models through activating interferon signaling and repressing the nuclear factor-?B signaling pathway

We explored the functional role of CLCF1 in osteoclastogenesis and bone loss associated with osteoporosis. Surprisingly, the administration of recombinant CLCF1 repressed excessive bone loss in ovariectomized mice and reduced the levels of local osteolytic lesions induced by a RANKL injection. Likewise, the addition of recombinant CLCF1 to RANKL-stimulated monocytes resulted in a significant suppression in the number of differentiated osteoclasts and their resorption activity on dentine slices. Overall design: RNA-seq for macrophages stimulated by macrophage colony-stimulating factor, receptor activator of nuclear factor kappa-b ligand and CLCF1