Raw data for "A mycobacterial Sec61 inhibitor disrupts lysosome function by blocking Vacuolar-ATPase biosynthesis"

This dataset contains the raw confocal and western blot images for the publication "A mycobacterial Sec61 inhibitor disrupts lysosome function by blocking Vacuolar-ATPase biosynthesis", Hall et al. European Journal of Cell Biology, 2026 which describes the impact of the bacterial toxin mycolactone on lysosomal function. Mycolactone inhibits the Sec61 translocon, causing mislocalisation of membrane and secretory proteins to the cytosol. Cells respond by upregulating the proteasome and autophagy pathways but the down regulation of the V-ATPase accessory proteins ATP6AP1 and ATP6AP2 leads to progressive loss of acidic lysosomes, limiting the ability of cells to restore homeostasis.本数据集包含发表于《欧洲细胞生物学杂志》2026年的论文《分枝杆菌Sec61抑制剂通过阻断空泡型ATP酶（Vacuolar-ATPase）生物合成破坏溶酶体功能》（作者：Hall等人）的原始共聚焦成像（confocal）与蛋白质印迹（Western Blot）图像。该论文阐述了细菌毒素分枝菌毒素（mycolactone）对溶酶体功能的影响：分枝菌毒素可抑制Sec61转运通道（Sec61 translocon），导致膜蛋白与分泌蛋白错误定位至细胞质基质；细胞会通过上调蛋白酶体与自噬通路做出应答，但空泡型ATP酶辅助蛋白ATP6AP1与ATP6AP2的下调会引发酸性溶酶体进行性丢失，进而限制细胞恢复内稳态的能力。