Expression data from primary astrocyte cell in CRTC3 KO and WT mice

CRTC3 is CREB regulated transcriptional co-activators and prominently expressed in astrocyte cells. CRTC3 plays a specific role in determining the social heirarchical ranking in mice by confirming social rank test in CRTC3 KO mice. Loss of CRTC3 in astrocyte induced neuronal change and caused exchanging protein expression pattern. We used microarrays to determine the astrocyte-derived factors that were regulated by CRTC3 and related with social dominance behaviours of CRTC3 KO mice. We identifed distinct factor of down-regulated gene during primary astrocyte cell in CRTC3 KO. Primary astrocyte were cultured from CRTC3 KO and WT mouse pups. To confirm the genes associated with cAMP responsive in CRTC3 KO astrocytes, WT and CRTC3 KO astrocyte cell were treated with vehicle and forskolin(FSK) for 0.5 and 2 hours.