Raw mass spectrometry data of recombinant human a1B/b2B-tubulin

We run about 5 μg of recombinant human a1B/b2B-tubulin on a 4-20% Tris-Glycine SDS-PAGE gel. The tubulin band is excised, reduced, alkylated, trypsinized, and analyzed by tandem LC-MS/MS.我们将约5微克的重组人源a1B/b2B微管蛋白上样至4-20% Tris-甘氨酸十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）凝胶并完成电泳。切取该微管蛋白条带，依次进行还原、烷基化、胰蛋白酶酶解处理，随后通过液相色谱-串联质谱（tandem LC-MS/MS）进行分析。