RNA Polymerase II binding profiles of HEK-293T cells exposed to hyperosmotic stress

Anti-Pol II ChIP-seq was performed to assess the redistribution of RNA Polymerase (Pol) II after hyperosmotic stress. Overall design: HEK-293T cells were treated with 80 mM KCl to induce hyperosmotic stress. Cells were crosslinked during the last 10 minutes of stress inductions using 1% formaldehyde. Chromatin immunoprecipitations were performed as described in Bieberstein et al. 2014 using 30 µL of anti-Pol II antibody (Cell Signaling D8L4Y).