Composition of chloroplast isolation buffers and wash buffers for modified high salt method, high salt plus saline Percoll method and sucrose gradient method.
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Both BSA and DTT were added just before the start of the experiment. Percoll gradient solutions consisted of wash buffer with Percoll at a final concentration of 70% (v/v) and 30% (v/v). Sucrose gradient solutions consisted of 50 mM Tris-HCl (pH 8.0), 25 mM EDTA and sucrose addition for a final concentration of 52% sucrose (w/v) and 30% (w/v) sucrose.
创建时间：
2014-01-02
