Loss of Capicua alters early T cell development and predisposes mice to T cell lymphoblastic leukemia/lymphoma. Mus musculus

We isolated RNAs from hematopoietic stem and progenitor cells (HSPCs). We compared two groups of mice: Cicflox/flox (control) and UBC-cre/ERT2; Cicflox/flox (knockout), two weeks post tamoxifen treatment. Following RNA isolation, RNAseq was performed and gene expression profiles were compared between controls and conditional knockouts. Overall design: Examination of gene expression in hematopoietic stem and progenitor cells (HSPCs) in two mouse groups.