Single-organism multimodal characterisation of eukaryotic plankton with Ukiyo-e-Seq

We used single-cell sequencing data and imaging to investigate Eukaryotic plankton from environmental marine samples collected from Coogee, NSW, Australia. Water was collected from the ocean, filtered and centrifuged to remove particles larger 1mm and smaller than 25um. A microscope-mounted cell picker was then used to image an organism in the sample and then transfer it to a well of a microtiter plate. Organisms were then analysed using single-cell rna seq.