Text S1 - Control of Virulence by Small RNAs in Streptococcus pneumoniae

Supplementary Materials. This file includes the following: Supplementary Methods; Strains used in this study (Table S1), Primers used in this study (Table S2); Coordinates of the additional regions of the TIGR4 genome containing the conserved motifs identified in Figure S4 (Table S3); Raw data from the Tn-seq screen (Table S4); Microarray analysis of attenuated sRNA mutants (Table S5); Proteins showing differences in abundance in the attenuated sRNA mutants compared to the parental TIGR4 (Table S6); Northern blots of sRNAs identified by RNA-seq (Figure S1); Differential expression of sRNA F13 in TCS response regulator knockouts (Figure S2); Motifs overrepresented in the sRNA sequences identified using the MEME software to identify motifs (Figure S3); qRT-PCR analysis of the genes flanking the sRNA knockouts (Figure S4); Adhesion and invasion of TIGR4R and the attenuated sRNA mutants to activated nasopharyngeal (Detroit) and endothelial (RBCEC6) cells (Figure S5); Representative 2D gel utilized for the proteomic analysis of TIGR4 with the individual spots being identified by circles (Figure S6). (DOC)