Crystal Structure of Dihydrofolate Reductase-Thymidylate Synthase from Cryptosporidium hominis Bound to 1843U89/NADPH/dUMP

Crystal Structure of Dihydrofolate Reductase-Thymidylate Synthase from Cryptosporidium hominis Bound to 1843U89/NADPH/dUMP Descriptor: 2'-DEOXYURIDINE 5'-MONOPHOSPHATE, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, S)-2-(5(((1,2-DIHYDRO-3-METHYL-1-OXOBENZO(F)QUINAZOLIN-9-YL)METHYL)AMINO)1-OXO-2-ISOINDOLINYL)GLUTARIC ACID, ... Authors: Anderson, A.C. Deposit date: 2004-02-17 Release date: 2004-05-18 Last modified: 2023-08-23 Method: X-RAY DIFFRACTION (2.87 Å) Cite: Two crystal structures of dihydrofolate reductase-thymidylate synthase from Cryptosporidium hominis reveal protein-ligand interactions including a structural basis for observed antifolate resistance. Acta Crystallogr.,Sect.F, 61, 2005

人隐孢子虫（Cryptosporidium hominis）二氢叶酸还原酶-胸苷酸合酶（dihydrofolate reductase-thymidylate synthase）与1843U89/烟酰胺腺嘌呤二核苷酸磷酸还原型（NADPH, dihydro-nicotinamide-adenine-dinucleotide phosphate）/2'-脱氧尿苷5'-单磷酸（dUMP, 2'-deoxyuridine 5'-monophosphate）结合的晶体结构描述：2'-脱氧尿苷5'-单磷酸、烟酰胺腺嘌呤二核苷酸磷酸还原型、(S)-2-(5-(((1,2-二氢-3-甲基-1-氧代苯并[f]喹唑啉-9-基)甲基)氨基)-1-氧代-2-异吲哚啉基)戊二酸，…… 作者：Anderson, A.C. 沉积日期：2004-02-17 发布日期：2004-05-18 最后修改日期：2023-08-23 实验方法：X射线衍射法（分辨率2.87 Å） 引用文献：两项针对人隐孢子虫二氢叶酸还原酶-胸苷酸合酶的晶体结构研究揭示了其蛋白质-配体相互作用模式，包括已观测到的抗叶酸耐药性的结构基础。发表于《Acta Crystallogr.,Sect.F》，第61卷，2005年。