Single cell 3' RNA sequencing of Foreign body granuloma

Although macrophage-epithelioid cell (EPC)-giant cell (GC) differentiation is acknowledged in foreign body reaction (FBR), the exact molecular features remain elusive. To discover the molecular profiles of EPC and GC, we analyzed mouse sponge and silk FBRs by integrating single-cell RNA-sequencing (scRNA-seq) and spatial sequencing. Chromium Single Cell 3ʹ (10x Genomics, Pleasanton, CA, USA) was used for library preparation of scRNA-seq according to the manufacturer’s protocol.