Juveniles were dissociated in pools of 15 polyps. Polyps were continuously labeled with EdU (100uM, 2%DMSO) before dissociation, and fixed using Trypsin/formaldehyde. Cells were stained with 1μg/ml Fx
As discussed in the main text, radiation effects were quantified by calculating the radiation-induced change (Qe) in CFU/ml, normalized by the initial cell concentration, as follows: Qe = (Xr(i) – Xc)
Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a
