LINE1 elements at Distal Junctions of rDNA repeats regulate nucleolar organization in human embryonic stem cells [RNA-seq]

In this study, we investigated whether RNA Pol II-mediated gene expression is affected by repression of DJ-LINE1. Guide RNAs targeting DJ-LINE1 or control genomic regions were transfected for 48h in CRISPRi naïve hESC. Overall design: Naive H9 hES cells were maintained RSeT. Cells were transfected with gRNA plasmids in RSeT media added with 10 nM DZNep and 5nM TSA (RSeT+DT). 48 hours later, cells were directly processed for RNA extraction and RNA-seq library prep.