Distinct roles of keratinocyte- and myeloid- MCPIP1 in maintaining skin homeostasis

The morphology of the skin changes upon depletion of MCPIP1 in cells of myeloid origin as well as in keratinocytes. The thicknesses of epidermal and hypodermal layers are significantly increased in mice with loss of epidermal MCPIP1, whereas loss of myeloid MCPIP1 exerts completely opposite effect. Both types of mice show contrary response to the stimulation with 12-O-tetradecanoylphorbol-13-acetate. Skin morphology and inflammatory phenotype of keratinocyte- and myeloid- double MCPIP1 knockout mice is similar to that of the single keratinocyte knockout of MCPIP1. In all, myeloid and epidermal MCPIP1 play significant but distinct roles in the modulation of skin-related processes. RNA-seq was performed to analyse transcriptomes of the whole skin lysates of 12-week Mcpip1EKO (keratinocyte-specific knockout), Mcpip1MKO (myeloid-specific knockout), Mcpip1EMdKO (keratinocyte and myeloid-specific knockout) and control mice.