RNA-seq analysis of hPSC-derived brain pericyte-like cells, hPSC-derived neural crest stem cells, and primary human brain pericytes

hPSCs were differentiated to brain pericyte-like cells through a neural crest stem cell intermediate and compared to primary human brain pericytes Overall design: 13 total samples were analyzed. Of these, 5 are hPSC-derived brain pericyte-like cells at D25 of the differentiation procedure (three biological replicates from the H9 cell line and one biological replicate each from the IMR90C4 and CS03n2 cell lines). Also included are H9 hPSCs, H9 hPSC-derived neural crest stem cells (NCSCs) at D15 of the differentiation procedure, H9 hPSC-derived NCSCs maintained to D55, and H9 hPSC-derived brain pericyte-like cells at D19, D22, and D45 of the differentiation procedure. For comparison, two samples of primary human brain pericytes are also included.