Gene silencing mediated by dsRNA reduces grey leaf spot disease in maize

The dataset contains the analysis of grey leaf spot disease severity scores for two spray-induced gene silencing (SIGS) trials performed on B73 maize infected with Cercospora zeina conidia. Disease severity was measured on a scale of S1 - S8, where S1 denotes no symptoms and S8 indicates the most severe disease symptoms. A positive disease control contained only C. zeina inoculum. The remaining plants were inoculated with C. zeina conidia and treated with either GFP double-stranded RNA (dsRNA) as a negative treatment control, or the test construct 3Cz dsRNA. In addition, this dataset includes the mean C. zeina conidial germination percentages calculated for C. zeina germinating in vitro on culture media (assessed with light microscopy) and in planta on maize leaves (assessed with scanning electron microscopy).

本数据集收录了在感染了由 Cercospora zeina 孢子诱导的灰斑病的大豆品种 B73 上进行的两种喷洒诱导的基因沉默（SIGS）试验的灰斑病严重程度评分分析。疾病严重程度按照 S1 至 S8 的标准进行测量，其中 S1 代表无病症，而 S8 则表示最严重的病症症状。阳性对照仅包含 C. zeina 接种物。其余植株均接种了 C. zeina 孢子，并分别用 GFP 双链 RNA（dsRNA）作为阴性处理对照，或测试构建的 3Cz dsRNA 进行处理。此外，该数据集还包含了在体外培养基上（采用光学显微镜进行评估）和在玉米叶片上（采用扫描电子显微镜进行评估）培养的 C. zeina 孢子的平均萌发百分比计算结果。