Single cell transcriptomic studies of mouse colonic lamina propria cells from wildtype or RORgtS182A mutant mice

The evolutionarily conserved serine 182 residue on ROR?t is phosphorylated. Here, we report that this residue is dispensable for thymic T cell development, but essential for maintaining proper balance of Th17-Treg populations in the colon. Single-cell RNA-seq (scRNA-seq) revealed that serine 182 residue on ROR?t is critical for restricting IL-1b-mediated Th17 activities and promoting anti-inflammation cytokine IL-10 production in Lymphoid tissue (LT)-like ROR?t+Foxp3+ regulatory T cells in inflamed tissues. Overall design: Two independent biological replicates in each condition were performed. In wildtype mice, results from DSS treated colon were compared to no treatment conditions. Results from RORgtS182A mutant mice were compared to those from wildtype mice in no treatment and DSS treatment conditions seperately.