Evaluation of splicing alterations in mice treated with RECTAS.

Aberrant pre-mRNA splicing events in tumor cells generate tumor-specific splice junctions, translating potential neoantigens. RNA-seq was performed to examine whether a small molecule compound RECTAS affect splice patterns followed by neoantigen repertoire in mouse colon, lung, and liver tissues. C67BL/6N mice of 7-weeks old were per os administered with 100 mg/kg body weight RECTAS in 0.5% carboxymethyl cellurose (CMC) or 0.5% CMC only, twice with 4 h interval. Individual tissue were collected for RNA extraction in total 8 h after the first administration. Extracted RNAs were applied for RNA-Seq.