Transcriptomic data for splenic neutrophils isolated from female NZBWF1/J lupus mice

Recent literature has shown that neutrophils exert significant influence on the course of disease progression in lupus, but there is currently controversy in the lupus field regarding a) whether neutrophils are predominantly protective or deleterious toward the propagation of auto-reactivity, b) the specific mechanism of neutrophil influence on adaptive immune dysregulation, and c) identity and origin of functionally abnormal neutrophil subsets in lupus. Here we used RNA sequencing of splenic neutrophils isolated from a murine lupus model (NZB/W) to characterize changes in the transcriptional program of neutrophils over the course of lupus progression. We find that the transcription of immunologically relevant genes change over the course of disease, and that lupus neutrophils early in disease show enrichment for transcripts associated with neutrophil precursors. These data provide the first detailed profile of the splenic neutrophil transcriptional program changes during the progression of lupus. UltraLoInput RNA sequencing of neutrophils isolated from the spleens of female NZBWF1/J lupus mice at three stages of disease (Ages: 14 weeks, 20 weeks, 26 weeks).近年来的研究表明，中性粒细胞（neutrophils）对狼疮（lupus）的疾病进展进程具有显著调控作用，但目前狼疮领域仍存在三方面争议：a) 中性粒细胞在自身反应性传播过程中主要发挥保护作用还是有害作用；b) 中性粒细胞影响适应性免疫失调（adaptive immune dysregulation）的具体机制；c) 狼疮患者中功能异常的中性粒细胞亚群（neutrophil subsets）的身份与起源。本研究针对小鼠狼疮模型（NZB/W）中分离得到的脾脏中性粒细胞开展RNA测序（RNA sequencing），以解析狼疮进展过程中中性粒细胞转录程序（transcriptional program）的动态变化。研究发现，与免疫相关的基因转录水平随疾病进程发生改变，且疾病早期的狼疮中性粒细胞富集了与中性粒细胞前体相关的转录本（transcripts）。本研究数据首次详细描绘了狼疮进展过程中脾脏中性粒细胞转录程序的变化图谱。本研究采用UltraLoInput RNA测序技术，对分别处于疾病三个阶段（周龄：14周、20周、26周）的雌性NZBWF1/J狼疮小鼠脾脏中分离的中性粒细胞进行测序。