Supporting data for "Regulation of viral overlapping genes and AAV vector engineering for antibody gene delivery overcoming the immunological barrier"

This study focuses on three objectives: (i) development of a system to regulate the viral overlapping genes, (ii) optimization of the AAV vector genome for antibody gene delivery, and (iii) AAV capsid engineering for overcoming the immunological barrier. The dataset includes three parts corresponding to the three chapters of thesis: Chapter3: intron-based regulation system to overlapping genes. This file includes three subfiles including an dataset Excel file, a powerpoint file with processed pictures and a zip file contianing plasmid maps used in this Chapter. Chapter4:optimization of mAb expression in gene therapy This file includes three subfiles including an dataset Excel file, a powerpoint file with processed pictures and a zip file contianing plasmid maps used in this Chapter. Chapter5: AAV capsid engineering for overcoming the immunological barrier. This file includes four subfiles including an dataset CSV file, a powerpoint file with processed pictures, a zip file contianing plasmid maps used in this chapter and a pdb subfiles containing predicted protein structure of the synbody described in this chapter.本研究聚焦三大核心目标：（i）开发可调控病毒重叠基因的系统，（ii）优化腺相关病毒（AAV）载体基因组以实现抗体基因递送，（iii）开展腺相关病毒衣壳工程以突破免疫屏障。本数据集包含与论文三个章节对应的三个部分：第三章：基于内含子的重叠基因调控系统。该章节对应的数据包含三个子文件，分别为数据集Excel文件、带处理后图片的PowerPoint演示文稿，以及本章所用质粒图谱压缩包。第四章：基因治疗中单克隆抗体（mAb）表达优化。该章节对应的数据包含三个子文件，分别为数据集Excel文件、带处理后图片的PowerPoint演示文稿，以及本章所用质粒图谱压缩包。第五章：用于突破免疫屏障的腺相关病毒衣壳工程。该章节对应的数据包含四个子文件，分别为数据集CSV文件、带处理后图片的PowerPoint演示文稿、本章所用质粒图谱压缩包，以及包含本章所述合成抗体（synbody）预测蛋白质结构的PDB（Protein Data Bank）文件。