Effect of everolimus on clear cell renal cell carcinoma (ccRCC) cell lines - parental and stem cells

The aim of this study was to compare effect of everolimus on growth of different renal cell carcinoma (RCC) populations and develop design for experiments to measure the early response of everolimus in clear cell RCC (ccRCC) cell lines including renal cancer stem cells. Gene expression profiling using microarray was performed to determine the early response to everolimus after 3 days of treatment with optimizied concentration of drug in two ccRCC cell lines 1) parental clear cell renal cell carcinoma ccRCC-PCSC (HKPCSC -human parental kidney cancer stem cells) and 2) ccRCC-CSC - clear cell renal cell carcinoma -cancer stem cells (HKCSC - human kidney cancer stem cells). Two condition experiment: 1) everolimus (E) treated and 2) untreated; 2 cell lines; parental clear cell renal cell carcinoma ccRCC-PCSC (P) and ccRCC-CSC - clear cell renal cell carcinoma -cancer stem cells (M). Biological replicates: 3, technical replicates 4