Optimization of Hydrolase Production (Dataset).pdf

The saccharification of biomass with hydrolases from fungi is considered as an efficient and sustainable approach to obtain fermentable sugars. The expression of these hydrolytic enzymes by fungi is mainly by a substrate-induced mechanism, which depends on prevailing environmental conditions. <i>Trametes polyzona, </i>a white rot fungus, is regarded as one of the most efficient producers of hydrolases for the hydrolysis of biomass. This study sought to examine and optimize cultivation conditions for the efficient production of hydrolases by <i>Trametes polyzona </i>(<i>T. polyzona</i>)<i>. </i>Hydrolase production by <i>T. polyzona</i> was examined using parametric optimization. Crude enzyme extracts obtained under varying cultivation conditions were profiled for endoglucanase, betaglucosidase, exoglucanase, xylanase and amylase activities to determine the optimal conditions for hydrolase production. Maximum hydrolase production was achieved by solid-state cultivation of <i>T. polyzona</i> on cassava peels at a moisture content of 60%, pH 6, supplemented with 0.3% urea and 0.03% Mg²⁺, and incubated at 30 ^oC for 14 days. A solid to liquid ratio of 1:2 was found to be ideal for the solid-liquid extraction of enzymes. This study therefore proves that optimization of cultivation conditions is an efficient approach to enhance the production of hydrolases required to breakdown lignocellulosic biomass by <i>T. polyzona.</i>