L-lactate supplementation influences toxin production of Clostridioides difficile via energy metabolism

Metabolomic and transcriptomic analysis of changes in the exponential and stationary phase of Clostridioides difficile after cultivation in casamino acids medium (reference) and supplemented with L-lactate and the connection to toxin production. Clostridioides difficile 630∆erm (DSM28645) was grown in a casamino acids medium (reference) and supplemented with 0.5 g/L L-lactate (Lac). Samples were taken at exponential growth (1/2 ODmax) and at the beginning of the stationary phase (ODmax).