miRNAs expression profile in bone marrow-derived PACs during ischemia-induced neovascularization in cardiovascular risk factors conditions

Using NGS, we report the impact of cardiovascular risk factors (CRF) including hypercholesteolemia, aging and smoking compare to healthy control mice, on the differential-expression profile of miRNAs in proangiogenic cells (PACs) isolated during ischemia-induced neovascularization in a mouse model of hindlimb ischemia. Data of total miRNA expression are presented as normalized copy number: read per millions of read (RPM). Hindlimb ischemia was surgicaly induced by feromal artery ablation and the animal was sacrified 2 day after ischemic surgery for PACs isolation. After 4 days of culture, PACs RNAs were extracted to perform NGS. Examination of the impact of CRF on miRNAs expression profile and modulation, in isolated PACs during ischemia-induced nerovascularization.