SKOV3 RBMS3 CLIP sequencing

RBMS3 is downregulated in various types of cancer, and they are associated with advanced tumor malignancy. For comprehensive understanding of the interaction between RBMS3 and their target RNAs, we exploited UV-crosslinking and immunoprecipitation (CLIP) to capture their in vivo binding to target RNAs. RBMS3-binding RNAs were identified in a ovarian cancer cell line SKOV3 using flag-magnetic beads. The result shows that RBMS3 preferentially binds to DKK3, AXIN1, BACH1 and NFAT5 through AUAA binding motif, which is consistent with our previous results.