Osteosarcoma tumors maintain intratumoral heterogeneity, even while adapting to environmental pressures that drive clonal selection

Osteosarcoma cells were grown in cell culture, as subcutaneous flank tumors, orthotpic primary tumors or experimental metastases to study the role of intra-tumor transcriptional heterogeneity on adaptations in tumor cells that faciliate tissue colonization. Overall design: single cell RNA-Seq profiles of human and murine osteosarcoma models (OS17, 143B, NCH-OS-2, NCH-OS-7, F420, K7M2) grown in cell culture, subcutaneous flank, primary tumor (bone) or metasatic lesions (lung)