Protease specificity profiling

We developed a high-throughput method for assaying protease cleavage specificity. Current methods are unable to reliably predict cleavage within the human proteome. However, our unique approach will screen all possible octa-peptide substrates in a single experiment by combining our mRNA display technology, next-generation sequencing, LC-MS/MS, and computational analysis. The data from up to millions of cleavage sequences will be compiled into a high-resolution specificity map. This map will facilitate the accurate prediction protease reactivity with human proteins or proteins from other organisms.