Phosphorylation of S.pombe Rap1 analysed by LC-MS/MS
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https://www.omicsdi.org/dataset/pride/PXD001841
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资源简介：
Identification and semi-quantitation of phosphorylation sites on Schizosaccharomyces pombe DNA-binding protein Rap1. A Rap1 construct was cultured in homozygous diploid temperature-sensitive pat1-114 mutant strain of S. pombe cells (time points: 3.5 h and 4.5h), and was purified by anti-PK immunoprecipitation. The resulting samples were run into a 1D SDS-PAGE gel with the target band excised, tryptically digested and analysed by LC-MS/MS using a Velos Orbitrap mass spectrometer.
创建时间：
2016-04-27
