Plk3 protein expression is reduced while its interaction with CIB1 is enhanced upon PMA treatment.

A) Western blots showing Plk3 protein expression in response to PMA treatment of Dami cells. Equal loading was assessed using HSC-70. D = DMSO as vehicle control; P = PMA; *p<0.05 vs mock. B) Densitometric data from (A) quantified and normalized to untreated control (CNTL) samples. †p<0.05 compared to CNTL; *p<0.05 compared to corresponding DMSO value. C) Representative Western blots of co-immunoprecipitation of CIB1 with Plk3 from Dami cell lysates. The blot was initially probed for CIB1, then stripped and reprobed for Plk3. Lys = Lysate, showing input. D) Western blot of lysates from Dami cells transfected with either Plk3 shRNA (Plk3sh) or empty vector (Mocksh) and probed for Plk3 and HSC-70. E) Representative flow cytometic histograms of DNA content of 3 day PMA treated Plk3sh and Mocksh cells.