Oxygen-balanced mixotrophic cultivation of Galdieria sulphuraria at lab scale under optimal and industrially relevant conditions

This dataset includes experimental data from lab-scale oxygen-balanced mixotrophic experiments in a stirred-tank cylindrical photobioreactor under chemostat and repeated bath modes. The experiments were conducted with the species Galdieria sulphuraria and tested the productivity and biomass yield on substrate under optimal conditions and under simulated tubular photobioreactor conditions with 3 different retention times.
Moreover, other parameters were also monitored along the experiments, including pigment content, protein content, C-phycocyanin content, quantum yield and the accumulation of coproporphyrin III in the supernatant. Glucose supply was controlled by changes in the DO signal. The aim of these experiments was to study the performance of oxygen-balanced mixotrophy under both optimized lab conditions and compare it to limiting industrial conditions.
Comparisons focused on biomass productivity, biomass yield on substrate and also stress markers. In addition, protein content under optimized conditions was also analyzed to determine the suitability of the species and the process for protein production. All the experiments where led by Pedro Moñino Fernández with the occasional collaboration of other group members and supervised by Marcel Janssen and Maria Barbosa.
The experiments were carried out at the chair group of Bioprocess Engineering at Wageningen University & Research during 2020 and 2021.