C3H/HeJ IAP-1Δ1 insertions absent from C3HeB/FeJ.
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https://figshare.com/articles/dataset/_C3H_HeJ_IAP_1_916_1_insertions_absent_from_C3HeB_FeJ_/1099054
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aTo detect both IAP insertion allele and the pre-insertion allele, the IAP5′LTR-F oligonucleotide is used in a 3′-primer assay with both the locus-specific forward and reverse primers. In cases where the product sizes of both alleles were identical, the IAP5′LTR-F+gene-F (or gene -R, depending upon IAP orientation) for the insertion allele and gene -F+gene -R primers are run separately for the pre-insertion allele.
创建时间:
2015-12-02



