(A) SDS-PAGE and Coomassie blue staining of the whole cell lysate corresponding to E. coli BL21 transformed with pCold-TF-AcLAP plasmid (lane 1), soluble fraction of the sonicated cell lysate (lane 2)
CONTROL OF ENZYME ACTIVITY BY AN ENGINEERED DISULFIDE BOND Descriptor: BETA-MERCAPTOETHANOL, CHLORIDE ION, T4 LYSOZYME Authors: Blaber, M, Matthews, B.W. Deposit date: 1994-06-20 Release date: 1994-08
Myosin I, an actin-dependent force-generating enzyme, has been purified from three mammalian sources: bovine adrenal medulla, adrenal cortex, and brain. The purification procedure includes extraction